| Catalog No. | Product Name | Specification |
| CRH00-3031 | ResiQuant® Ready-to-use CHO HCP ELISA Kit 2G | 48T |
| CRH00-3032 | ResiQuant® Ready-to-use CHO HCP ELISA Kit 2G | 96T |
Product Overview
The ResiQuant® CHO HCP ELISA KIT-FAST is a ready-to-use and quantitative ELISA kit. This kit is specifically intended for the detection of host cell protein contaminants in products derived from Chinese Hamster Ovary (CHO) expression. The limit of detection (LOD) for this kit is 0.5 ng/mL, and the limit of quantitation (LLOQ) is 1.0 ng/mL. It is suitable for use throughout the manufacturing process as well as for product release.
● Sensitive measurement: the limit of detection (LOD) is 0.5 ng/ml and the limit of quantification (LLOQ) is 1.0 ng/ml; spiking recovery rate qualified, within the range of 70%-130%
● High Antibody Coverage: Characterized by Antibody Affinity Extraction (AAE) and Mass Spectrometry, with 84.8% coverage, highly risk impurities also included
● Easy to use: Directly use without dilution
Specifications & Data
| LOD | 0.5 ng/ml | Storage | 2℃ to 8℃ |
| LLOQ | 1.0 ng/mL | Shipping Condition | Dry Ice |
| Recovery Rate | 70%~130% | Host Cell | CHO |
| Intra-batch | ≤15% | Shelf Life | 12 months |
| Inter-batch | ≤20% | Quantity | 50/100 reactions |
| Accuracy | RSD≤20% | Includes | Assay Kit only |
Fig 1. Data analysis is suggested using a nonlinear fit of the sigmoidal curve generated by a range of standard concentrations. |
Fig 2. Antibody coverage rate was evaluated by AAE and LC-MS. CHO HCPs and AAE purified CHO HCPs were applied to LC-MS as pre-AAE and Post-AAE respectively. The sum of the identified post-AAE and pre-AAE HCPs was recognized as the total number of the whole HCPs. The recovery rate was calculated using the number of the whole HCPs divided by the number of post-AAE HCPs. |
Documents
MSDS_CRP303X
Manual_CRH303X
Application
ExCell Bio has built an advanced culture media factory with large-scale, informationization and internationalization. We can provide international-leading high-performance and high-quality classical cell culture Fetal bovin serum. We can provide different
"The therapeutic use of stem cells as potential therapies for a variety of diseases has been widely explored, and the number of clinical trials conducted using mesenchymal stem cells has grown exponentially in the past few years.Stem cells have uniqu
In the field of cell and gene therapy (CGT), Excell Bio provides you with end-to-end cell and gene therapy solutions to help you develop therapies that can save lives. Our mission is to build Bridges to help you reach your next milestone, to help you prog
